The Effect of Gamithromycin on Smooth Muscle of Rat Uterus In Vitro

Effect of gamithromycin on rat uterus contractility

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Authors

  • Tolga Trak 1Kalecik District Directorate of Agriculture and Forestry, Kalecik /Ankara
  • Ebru Yıldırım Kırıkkale University Veterinary Faculty Pharmacology and Toxicology Department Yahşihan / Kırıkkale
  • Begüm Yurdakök Dikmen 3Ankara University, Veterinary Faculty, Department of Pharmacology and Toxicology, Dışkapı / Ankara

Keywords:

Gamithromycin, in vitro, oxytocin, rat, uterus smooth muscle.

Abstract

The purpose of this study was to investigate the effect of gamithromycin on rat uterus smooth muscles and to evaluate the possible mechanism of action. Forty-four uterine tissues isolated from 16 female Wistar rats weighing 200-250 grams were used in the experiments. In the preliminary experiments, gamithromycin was tested at concentrations of 10-7 M, 10-6 M and 10-5 M; where 10-5 M was selected for the experiments. In Group 1, the uterus segments were treated for 10 min with 10-5 M gamithromycin following 10 min control contractions. In Group 2, the effect of 10-5 M gamithromycin over 2.5 mIU / mL oxytocin contraction was evaluated. In Group 3, the effect of 10-5 M gamithromycin was evaluated over 10-8 M atropine incubation. In Group 4, 10-5 M gamithromycin was applied for 10 min followed by 0.0625 μg/ml of cloprostenol application. The same protocols were applied for dimethylsulfoxide (DMSO) as control. Frequency, average amplitude and peak amplitude values ​​of the contractions were assessed. In Group 1, gamithromycin in were shown to increase the contractility at 10-5 M significantly (P≤0.01); while no statistically significant difference was observed in comparison to DMSO (P> 0.05). For the other tested groups, no statistically important difference were observed (P> 0.05). The nonsignificant difference of the results of this study can be attributed to the chemical form of gamithromycin and the concentration used. In order to be able to fully assess the effects and possible mechanism of gamihtromycin on the uterine smooth muscle, higher gamithromycin concentrations should be studied and, if possible, further studies should be performed with different agonist and antagonist agents.

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Published

2019-12-18

How to Cite

Trak, T., Yıldırım, E., & Yurdakök Dikmen, B. (2019). The Effect of Gamithromycin on Smooth Muscle of Rat Uterus In Vitro: Effect of gamithromycin on rat uterus contractility. International Journal of Veterinary and Animal Research (IJVAR), 2(2), 46–50. Retrieved from https://ijvar.org/index.php/ijvar/article/view/324

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Section

Research Articles