Evaluation of antifungal activity of nanobiosilver particles to treat Candida albicans releated urogenital infections in female rat model

Abstract

Candida species are the most common encountered agent of fungal infections. Catheter related infections affect over one million patients in Europe and US annually. Candida species infections are responsible for over 63 % of fungal infections in veterinary practice. The aim of this study was to investigate the therapeutic efficacy of nanoparticles in urinary catheter related cystitis and vulvo vaginitis of C.albicans, which is common for human and veterinary medicine is very hard to treat. Thirty healthy adult female Wistar rats were used in the study. The rats were divided into three groups; experimental group with C.albicans inoculation and treatment (n = 10) and positive control group C.albicans inoculations without treatment (n=10) and negative control group with urinary catheter placed without any pathogen inoculation (n=10). Nanobiosilver was administrated after the establishment of infection. Vulvovaginal and urinary bladder tissues collected at necropsy were process for Gomori staining histopathology and electron microscopy. Rats received inoculation exhibited significant outcomes associated with fungal infections compare to negative control rats. Results of control group were between physiological limits. C.albicans was detected in urine samples at 72^nd hours after inoculation in experimental group but not observed at 7^th day in animals treated with nanobiosilver. In conclusion, due to its safety, efficacy and lack of systemic effects, nanobiosilver could be an excellent alternative for the initial treatment of catheter related candidiasis in veterinary medicine.