International Journal of Veterinary and Animal Research (IJVAR) 2023-09-06T00:14:37+03:00 Assoc.Prof.Dr.Husamettin Ekici Open Journal Systems <p>International Journal of Veterinary and Animal Research (IJVAR) accepts original research, reviews, clinical trials, case reports and correspondence. <strong>***As of January 2019, manuscripts written only in English has been processed for evaluation and publication.***</strong></p> <ul> <li class="show">​Animal feed and nutrition</li> <li class="show">Anatomy</li> <li class="show">Animal Behavior</li> <li class="show">Animal biotechnology</li> <li class="show">Animal breeding</li> <li class="show">Animal Diseases</li> <li class="show">Animal Feed</li> <li class="show">Animal husbandry</li> <li class="show">Animal nutrition</li> <li class="show">Animal Nutrition&nbsp; and Welfare</li> <li class="show">Animal parasitology</li> <li class="show">Animal Immunology</li> <li class="show">Animal physiology</li> <li class="show">Animal Reproduction</li> <li class="show">Animal Vaccine</li> <li class="show">Animal Viruses</li> <li class="show">Biological science</li> <li class="show">Diseases Companion and Wild Animals</li> <li class="show">Equine​</li> <li class="show">Ethology</li> <li class="show">Fisheries</li> <li class="show">Food Safety</li> <li class="show">Infectious diseases</li> <li class="show">Marine Science</li> <li class="show">Pathology</li> <li class="show">Pharmacological medicine</li> <li class="show">Transgenic animal production</li> <li class="show">Veterinary Clinical Research</li> <li class="show">Veterinary medicine</li> <li class="show">Veterinary Parasitology</li> <li class="show">Veterinary Pharmacology and Toxicology</li> <li class="show">Veterinary science</li> <li class="show">Veterinary Technician</li> <li class="show">Zoonotic Disease</li> <li class="show">Zootechny</li> </ul> Traditional Chinese Herbal Medicine Fufang E’Jiao Jiang as Supportive Treatment in Anaplasmosis Management of a British Shorthair Cat 2023-04-20T09:40:55+03:00 Pramita Rabiulfa Palagan Senopati Sewoyo Made Santi Purwitasari I Gede Soma I Gusti Made Krisna Erawan <p>Anaplasmosis, caused by obligate gram-negative intracellular parasitic bacteria <em>Anaplasma</em> sp., is a serious disease affecting both domestic and wild animals. In summary, Anaplasmosis is a serious disease that requires prompt diagnosis and aggressive treatment. The presented case highlights the importance of a comprehensive treatment plan in managing the disease and the potential benefit of incorporating traditional Chinese herbal medicine into the treatment regimen. In this case, a female British Short Hair cat, weighing 2.29 kg and aged one year and eight months, presented with symptoms including lethargy, loss of appetite, and sneezing. Physical examination of the cat revealed several abnormalities including an increase in body temperature, capillary refill time of more than two seconds, slow skin turgor, pale eye mucosa and gums, and rales in breathing. Routine hematology examination results indicated the presence of hypochromic macrocytic anemia and thrombocytopenia, while blood biochemistry results revealed an increase in globulin and aspartate aminotransferase levels and a decrease in the albumin/globulin ratio. The examination of blood smear preparations showed intracytoplasmic inclusions (morula) of the blood parasite <em>Anaplasma</em> sp. The cat received a comprehensive treatment regimen including an infusion of 0.9% sodium chloride, doxycycline antibiotics, acetylcysteine mucolytic drugs, ornipural® hepatoprotector vitamin, and traditional Chinese herbal medicine <em>Fufang E'Jiao Jiang</em> (FEJ). Despite seven days of hospitalization, the cat's condition did not improve, and the hematocrit examination results continued to decline. As a result, a blood transfusion was performed, and the FEJ treatment was continued. After the transfusion, the cat's condition improved significantly, and on the 20<sup>th</sup> day, the cat was discharged from the hospital. Based on the results of the hematological examination which improved after blood transfusions, the supportive treatment with FEJ was quite promising. In addition, this study is a preliminary report and it is suggested to carry out large-scale clinical trials with various doses.</p> 2023-09-06T00:00:00+03:00 Copyright (c) 2023 International Journal of Veterinary and Animal Research (IJVAR) Comparison of Passive Transfer Between Lambs Receiving Colostrum by Natural Suckling and Lambs Given Colostrum by Bottle 2022-12-21T13:59:21+03:00 Celal Eğdir Naci Öcal <p>Since the syndesmochorial placental structure of sheep does not allow the passage of large molecules from mother to offspring in intrauterine life, lambs are born hypogammaglobulinemic. The aim of this study is to compare passive transfer of immunity, neonatal morbidity and mortality, and live weight gains at day 120 between lambs receiving colostrum by suckling naturally and lambs given colostrum by bottle. 61 (n=61) lambs born to 43 sheep were randomly divided into two groups. One of the groups (NL group) received the desired amount of colostrum from the mother as they desired after birth. For the other group (BL group), access to the mothers' udder was restricted by a pouch after the lambs were born. BL group lambs were given colostrum roughly 8% of their birth weight by bottle as soon as possible after birth. Afterwards, colostrum freshly obtained from the mother was given to these lambs <em>ad libitum</em> every 4-6 hours during the first 24 hours. Approximately 50 ml of colostrum was taken into sterile containers from all sheep included in the study within the first 30 minutes after birth. Blood samples were taken from lambs for IgG analysis 36-48 hours after the first colostrum intake. Both serum and colostrum IgG analyses were performed by Radial Immunodiffusion (RID) method. Serum IgG concentrations of lambs in the BL group (3155.41±1245.25 mg/dl) were found to be higher than serum IgG concentrations of lambs in the NL group (2097.02±1213.07 mg/dl). When the results were compared using Independent Samples T test, the difference between the two groups was very statistically significant (p&lt;0.001). Neonatal morbidity and mortality were higher in lambs in the NL group (46.6%, 23.2%, respectively) than in lambs in the BL group (22.6%, 12.9%, respectively). Failure of Passive Transfer of Immunity (FPT) in lambs in the NL group (30%) was much higher than in lambs in the BL group (6.45%). Although the average Live Weight (LW) of lambs in the BL group (40.70 +7.84 kg) was higher than the average of lambs in the NL group (36.47+10.46 kg), this difference was statistically insignificant. In conclusion, giving lambs colostrum by bottle as soon as possible after birth without waiting for lambs to naturally receive colostrum from the mother results in better Passive Transfer and, accordingly, lower neonatal morbidity and mortality, and thus can help reduce economic loss in sheep breeding enterprises.</p> 2023-09-06T00:00:00+03:00 Copyright (c) 2023 International Journal of Veterinary and Animal Research (IJVAR) Taurine Reduced Reproductive Performance and DNA Damage Induced by Lead in Drosophila melanogaster 2023-03-14T21:02:35+03:00 Fahriye Zemheri Navruz Özge Çeliktaş Köstekçi Sinan İnce <p>Lead is one of the most important pollutants in the environment and food chain. This heavy metal causes serious health risks, especially cancer, in humans and animals. Taurine is an amino acid that can be synthesized mainly from methionine and cysteine and is used especially in the food industry. This study investigated the possible protective role of taurine on reproductive performance and DNA damage in <em>Drosophila melanogaster</em> exposed to lead. Lead and taurine were added to the broth of <em>D. melanogaster</em> at 100 µM and 3 mM, respectively, for 15 days. The Comet method was used for the determination of DNA damage. It was found that there was raise in DNA damage in the lead-administered groups, whereas taurine reduced the DNA damage induced by lead. In addition, it was determined that lead caused a decrease, whereas taurine had a positive effect on reproductive performance. As a result, it was determined that taurine prevented the negative effects of lead on <em>D. melanogaster</em> and showed protective properties.</p> 2023-09-06T00:00:00+03:00 Copyright (c) 2023 International Journal of Veterinary and Animal Research (IJVAR) Determination of Neonicotinoid Residues in Honey Samples Collected from The East-Southeast Anatolia Region of Türkiye 2023-03-12T23:42:30+03:00 Hikmet Dinç Nurullah Özdemir Fuat Serkan Kapucuk <p>In this study, 30 honey specimens were collected from some provinces of East-Southeast Anatolia to investigate the residue of neonicotinoid type insecticide. After the collected honey specimens were extracted with the liquid-liquid extraction method, the analyzes were carried out in a High-Pressure Liquid Chromatography Mass Spectrometry (HPLC-MS/MS) device. According to the analysis results, Acetamiprid in honey samples was observed to be in the range of 0.93-4.98 μg/kg. These results were found to be below the Maximum Residue Limit (MRL) value of 5 μg/kg according to FAO/WHO and Turkish Food Codex (TGK). It was determined that the results obtained for other analytes were insignificant and were in accordance with the residue limits of neonicotinoids in honey.</p> 2023-09-06T00:00:00+03:00 Copyright (c) 2023 International Journal of Veterinary and Animal Research (IJVAR) Investigation of Some Antioxidant Enzyme Levels in Subclinical Mastitis in Dairy Cows at First 100 Days of Lactation 2023-06-20T17:07:46+03:00 Taha Burak Elifoğlu Ayhan Baştan Bülent Baş <p>The purpose of this study is to investigate relationship between milk somatic cell count (SCC), total bacteria count (TBC), milk glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and serum paraoxonase (PON) levels, to identify the role of these parameters in subclinical mastitis at cows in the first 100 days of lactation. Animal material of this study consists of 100 Holstein dairy cows which were raised in a farm in Ankara province. Selected animals were hosted under same conditions and had suitable feeding regimen for appropriate period and were in the first 100 days of lactation. Cows which have less than 200x10<sup>3</sup> somatic cell counts in two different analysis which performed 7-10 days apart considered as healthy (n=50), and which had 200x10<sup>3</sup> somatic cell counts in two different counts performed 7-10 days apart considered as subclinical mastitis (n=50). Serum paraoxonase (PON) activity was lower in cows with subclinical mastitis than healthy ones (p&lt;0.001), and GSH-Px activity was higher in cows with subclinical mastitis (p&lt;0.001). It was concluded that the changes in milk SOD and GSH-Px levels are affected by the antioxidant system of the udder in subclinical mastitis, that formed oxidative stress and because of that serum PON and milk somatic cell and total bacteria have negative correlation. In conclusion, it was pointed out that serum PON activity can be used as a marker in the diagnosis of subclinical mastitis and evaluation of the antioxidant status of the udder. Also, it was stood out to importance of udder antioxidant mechanisms to understand pathogenesis of subclinical mastitis especially occurs in first 100 days of lactation.</p> 2023-09-06T00:00:00+03:00 Copyright (c) 2023 International Journal of Veterinary and Animal Research (IJVAR) Deep Dive of Vitamin D among Respiratory Diseased and Healthy Calves 2023-07-06T17:40:52+03:00 Deniz Alıç Ural <p>The requirement for vitamin D intake of dairy cattle has been better understood within the past few years and is well accepted by dairy producers and veterinary surgeons. In our country, as to the present author knowledge. In our country, as to the present author knowledge whether current recommendations and practices for supplemental vitamin D are meeting the needs of dairy cattle, however, is not well known. Circulatory vitamin D status of calves has been truly denoted via 25-hydroxyvitamin D [25(OH)D] metabolite levels in serum/plasma, with a concentration of 30 ng/mL proposed as a lower threshold for sufficiency. The present author was unaware of finding documented reports regarding vitamin d and its alterations among calves with respiratory distress. In the present study 16 calves (Group 1) were presenting clinical signs of respiratory problems and other 12 (Group 2) were selected as healthy control group. All necessary blood samples were taken were analyzed by Savant Fluorescent Immunoassay Device by use of Savant 25(OH)D test kits. Vitamin D (ng/mL) (X ̅ ± SE) among healthy calves and calves with pneumonia were detected as 63.21 ± 5.63 vs. 23.80 ± 2.75 with a statistical significance (p ≤0,001). In summary it should not be unwise to draw conclusion that pulmonary defence against respiratory infections should involve Vitamin D supplementation at appropriate dosages among calves.</p> 2023-09-06T00:00:00+03:00 Copyright (c) 2023 International Journal of Veterinary and Animal Research (IJVAR) The Alterations of Erythrocyte Osmotic Fragility and Hematological Parameters in Puppies with Parvoviral Enteritis in Kirikkale, Türkiye 2023-08-07T13:39:18+03:00 Ruhi Kabakci Erdal Kara <p>Canine parvoviral enteritis (CPVe) is an important infectious disease that has pretty high morbidity and mortality rates in puppies, which leads to vomiting and diarrhea range from mucoid to hemorrhagic. The objective of this study to investigate the changes in erythrocyte osmotic fragility (EOF) and hematological parameters in dogs with CPVe. Sixteen healthy and twenty-seven infected male and female dogs identified by the CPV antigen rapid-test kit at ages 2-6 months old were used to collect blood samples. A complete blood count and EOF were measured from the samples. Findings of hemogram showed that WBC, RBC, and HCT values were significantly lower in infected dogs (the p-value of the first one is p&lt;0.001, other two ones are p&lt;0.05) compared to healthy controls. The infected dogs had non-significantly lower NEU, EOS, and PLT values, and higher LYM, MON, MCV, MCH, and MCHC values than control group (p&gt;0.05). Although the hemolysis rate of infected dogs' erythrocytes at the concentrations of 0.4 - 0.1 % NaCl were higher than that of healthy dogs, this difference was statistically significant at only 0.3% NaCl (p&lt;0.05). In addition, the median corpuscular fragility (MCF), equal to the NaCl concentration that lyses 50% of erythrocytes, of dogs with CPVe (0.42%) was higher than the MCF values of the control group (0.39%), but not statistically significant (p&gt;0.05). In conclusion, besides the hematological findings revealed that WBC, RBC, and HCT values were significantly lower in dogs with CPVe than that in healthy dogs in this study, it was reported for the first time that canine parvovirus significantly increased osmotic fragility of RBCs at 0.3 % NaCl concentration compared to the control group. We concluded that to consider of these, particularly affected, blood parameters may be useful for determining the prognosis of CPVe.</p> 2023-09-06T00:00:00+03:00 Copyright (c) 2023 International Journal of Veterinary and Animal Research (IJVAR)