Kaynak Alabalığının Salvelinus fontinalis Semeninin Bakteriyel Florasının Belirlenmesi
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Bacterial count, brook trout, Salvelinus fontinalis, semenAbstract
Experiments were designed to evaluate bacterial flora in semen culture of brook trout (Salvelinus fontinalis). Herein, semen samples were
collected from fish and standard microbiology techniques were processed for bacterial flora. The samples were serially diluted in physiological
saline (NaCl: 0.85%). Aliquots of 0.1 ml of each dilution were spread-plated onto Plate Count Agar (PCA) (Total Bacteria Count), Rose Bengal
Agar (RBC) (Yeast-Mold Count), VRBD (Enterobacteriaceae count) and Mannitol Salt Agar (MSA) (Micrococcus/Staphylococcus count).
Micrococcus/Staphylococcus group bacteria (11.11%) were counted from brook trout (S. fontinalis) semen. Total bacteria count varied between
103-105 cfu ml-1 in semen.