Investigation of Some Antioxidant Enzyme Levels in Subclinical Mastitis in Dairy Cows at First 100 Days of Lactation
Abstract views: 83 / PDF downloads: 64Keywords:
Dairy cow, glutathione peroxidase, oxidative stress, paraoxonase, subclinical mastitisAbstract
The purpose of this study is to investigate relationship between milk somatic cell count (SCC), total bacteria count (TBC), milk glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and serum paraoxonase (PON) levels, to identify the role of these parameters in subclinical mastitis at cows in the first 100 days of lactation. Animal material of this study consists of 100 Holstein dairy cows which were raised in a farm in Ankara province. Selected animals were hosted under same conditions and had suitable feeding regimen for appropriate period and were in the first 100 days of lactation. Cows which have less than 200x103 somatic cell counts in two different analysis which performed 7-10 days apart considered as healthy (n=50), and which had 200x103 somatic cell counts in two different counts performed 7-10 days apart considered as subclinical mastitis (n=50). Serum paraoxonase (PON) activity was lower in cows with subclinical mastitis than healthy ones (p<0.001), and GSH-Px activity was higher in cows with subclinical mastitis (p<0.001). It was concluded that the changes in milk SOD and GSH-Px levels are affected by the antioxidant system of the udder in subclinical mastitis, that formed oxidative stress and because of that serum PON and milk somatic cell and total bacteria have negative correlation. In conclusion, it was pointed out that serum PON activity can be used as a marker in the diagnosis of subclinical mastitis and evaluation of the antioxidant status of the udder. Also, it was stood out to importance of udder antioxidant mechanisms to understand pathogenesis of subclinical mastitis especially occurs in first 100 days of lactation.
References
Andrei S, Matei S, Zinveliu D, Pintea A, Bunea A, Ciupe S, Groza I. 2010. Correlations between antioxidant enzymes activity and lipids peroxidation level in blood and milk from cows with subclinical mastitis. Bulletin UASVM, 67(1): 6–11.
Atakisi O, Oral H, Atakisi E, Merhan O, Pancarci SM, Ozcan A, Marasli S, Polat B, Colak A, Kaya S. 2010. Subclinical mastitis causes alterations in nitric oxide, total oxidant and antioxidant capacity in cow milk. Res Vet Sci, 89(1): 10–13.
Armstrong D. 2008. Advanced protocols in oxidative stress I. Human Press. Herts.
Atroshi F, Parantainen J, Sankari S, Österman T. 1986. Prostaglandins and glutathione peroxidase in bovine mastitis. Res Vet Sci, 40: 361–366.
Baştan A. 2013. İneklerde meme sağlığı ve sorunları. Kardelen, Ankara.
Blowey RW, Edmondson P. 2010. Mastitis control in dairy herds. Cabi, Oxon.
Boulanger V, Zhao X, Lacasse P. 2002. Protective effect of melatonin and catalase in bovine neutrophil-induced model of mammary cell damage. J Dairy Sci, 85: 562–569.
Bradley AJ. 2002. Bovine mastitis: an evolving disease. Vet J, 164: 116–128.
Colakoglu HE, Kuplulu O, Vural MR, Kuplulu S, Yazlik MO, Polat IM, Oz B, Kaya U, Bayramoglu R. 2017. Evaluation of the relationship between milk glutathione peroxidase activity, milk composition and various parameters of subclinical mastitis under seasonal variations. Vet Arh, 87: 557–570.
Dimri U, Sharma MC, Singh SK, Kumar P, Jhambh R, Singh B, Bandhyopadhyay S, Verma MR. 2013. Amelioration of altered oxidant/antioxidant balance of Indian water buffaloes with subclinical mastitis by vitamins A, D3, E, and H supplementation. Trop Anim Health Prod, 45(4): 971–978.
Erskine RJ, Eberhart RJ, Hutchinson LJ, Scholz RW. 1987. Blood selenium concentrations and glutathione peroxidase activities in dairy herds with high and low somatic cell counts. J Am Vet Med Assoc, 190: 1417–1421.
Fuhrman B. 2012. Regulation of hepatic Paraoxonase-1 expression. Journal of Lipids, 2012: 1-5.
Ghasemian KO, Safi S, Rahimi FA, Bolourchi M. 2011. Study of the relationship between oxidative stress and subclinical mastitis in dairy cattle. IJVR, 12(4): 350-353.
Hamann J. 2005. Diagnosis of mastitis and indicators of milk quality. Mastitis in dairy Production: Current Knowledge and Future Solutions, 82–90.
Hamed H, El Feki A, Gargouri A. 2008. Total and differential bulk cow milk somatic cell counts and their relation with antioxidant factors. C R Biol, 331: 144–151.
Holbrook J, Hicks CL. 1978. Variation of superoxide dismutase in bovine milk. J Dairy Sci, 61: 1072–1077.
Kannan K, Jain SK. 2000. Oxidative stress and apoptosis. Pathophysiology, 7: 153–163.
Komine K, Kuroishi T, Komine Y, Watanabe K, Kobayashi J, Yamaguchi T, Kamata S, Kumagai K. 2004. Induction of nitric oxide production mediated by tumor necrosis factor alpha on staphylococcal enterotoxin C-stimulated bovine mammary gland cells. Clin Vaccine Immunol, 11: 203–210.
Kovacic M, Samardzija M, Duricic D, Vince S, Flegar-Mestric Z, Perkov S, Gracner D, Turk R. 2019. Paraoxonase-1 activity and lipid profile in dairy cows with subclinical and clinical mastitis. J Appl Anim Res, 47: 1–4.
Kulka M. 2016. A review of paraoxonase 1 properties and diagnostic applications. Pol J Vet Sci, 19: 225–232.
Kuplulu S, Vural MR. 2016. Büyük Ruminantlarda Meme Sağlığı Kontrol Programları, in: Kaymaz M, Fındık M, Rişvanlı A, Köker A. (Eds.), Evcil Hayvanlarda Meme Hastalıkları. Medipress, Ankara, Turkey, pp. 261–294.
Lauritzen B, Lykkesfeldt J, Friis C. 2003. Evaluation of a single dose versus a divided dose regimen of danofloxacin in treatment of Actinobacillus pleuropneumoniae infection in pigs. Res Vet Sci, 74: 271–277.
Lievaart JJ, Kremer WDJ, Barkema HW. 2007. Comparison of bulk milk, yield-corrected, and average somatic cell counts as parameters to summarize the subclinical mastitis situation in a dairy herd. J Dairy Sci, 90: 4145–4148.
Lykkesfeldt J, Svendsen O. 2007. Oxidants and antioxidants in disease: oxidative stress in farm animals. Vet J, 173: 502–511.
Matei ST, Groza I, Bogdan L, Ciupe S, Fit N, Andrei S. 2011. Correlation Between Mastitis Pathogenic Bacteria and Glutathione Peroxidase Activity in Cows Milk. Bulletin UASVM, 68(1): 221–225.
Mazur A. 1946. An enzyme in animal tissues capable of hydrolyzing the phosphorus-fluorine bond of alkyl fluorophosphates. J Biol Chem, 164(1): 271–289.
Nedic S, Vakanjac S, Samardzija M, Borozan S. 2019. Paraoxonase 1 in bovine milk and blood as marker of subclinical mastitis caused by Staphylococcus aureus. Res Vet Sci, 125: 323–332.
Olechnowicz J, Jaśkowski JM. 2012. Somatic cells count in cow’s bulk tank milk. J Vet Med Sci, 74(6): 681–686.
Paglia DE, Valentine WN. 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Cm Med, 70(1): 158–169.
Pei J, Pan X, Wei G, Hua Y. 2023. Research progress of glutathione peroxidase family (GPX) in redoxidation. Front Pharmacol, 14: 1–14.
Rehman SB, Hussain I, Rashid SM. 2017. Assessment of antioxidant profile in subclinical and clinical mastitis in dairy cattle. J Entomol Zool Stud, 5: 1023–1025
Rizzo A, Roscino MT, Binetti F, Sciorsci RL. 2012. Roles of Reactive Oxygen Species in Female Reproduction. Reprod Domest Anim, 47: 344–352.
Schukken YH, Wilson DJ, Welcome F, Garrison-Tikofsky L, Gonzalez RN. 2003. Monitoring udder health and milk quality using somatic cell counts. Vet Res, 34(5): 579–596.
Shekhanawar M, Shekhanawar SM, Krisnaswamy D, Indumati V, Satishkumar D, Vijay V, Rajeshwari T, Amareshwar M. 2013. The role of paraoxonase-1 activity as an antioxidant in coronary artery diseases. J Clin Diagn Res, 7(7): 1284-1287.
Turk R, Piras C, Kovacic M, Samardzija M, Ahmed H, De Canio M, Urbani A, Mestric ZF, Soggiu A, Bonizzi L, Roncada P. 2012. Proteomics of inflammatory and oxidative stress response in cows with subclinical and clinical mastitis. J Proteomics, 75(14): 4412–4428.
Yang FL, Li XS, He BX, Yang XL, Li GH, Liu P, Huang QH, Pan XM, Li J. 2011. Malondialdehyde level and some enzymatic activities in subclinical mastitis milk. African J Biotechnol, 10(28): 5534–5538.
Yang FL, Li XS. 2015. Role of antioxidant vitamins and trace elements in mastitis in dairy cows. J Adv Vet Anim Res, 2: 1–9
Younus H. 2018. Therapeutic potentials of superoxide dismutase. Int J Health Sci, 12(3): 88–93.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 International Journal of Veterinary and Animal Research (IJVAR)
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
