Otic Microbiome Transplantation in a Dog with Recurrent Otitis Externa: Unraveling Allergic Secrets with Break Through Treatment Methodology

Abstract

Canine otitis externa (OE) is a common inflammatory disease that is frequently complicated by secondary bacterial and/or yeast infections. The otic microbial population is more complex than appreciated by cytological methods and aerobic culture alone. In the present case report a dog with recuurent OE and newly diagnosed otitis media was enrolled. Due to previous unsuccessful treatment trials with immunosupressive agents and antibacterial choices, with polypharmacy, we decided to perform manupilation of the microbiome in an attempt to investigate otic microecology. For this purpose first author gave his opinion for frontier knowledge and several years of experience for microbiome transplantation. Given literature data regarding ear canal microbiome among dogs with OE, is easily distinguishable to that of healthy ones, we decided to perform otic microbiome transplantation (omT). Microbiome samples were taken prior to and thereafter 10th days of omT, were collected and sent to MiDOG Limited Liability Company (LLC) service for next generation sequencing. Following omT, clinical signs were resolved and regressed, even starting on day 3 with satisfactory results (exudate was disappeared). Microbiome analytes showed Porphyromonas cangingivalis, Peptoclostiridium sp., Pasteurella canis, Malassezia pachydermatis and Aspergillus sp. with increased relative abundances, whereas no more pathogen were evident 10 days after omT. Pathogens were switched and substituted with new microorganisms with potential probiotic facilities. Observed species were increased following omT [prior to omT 47 vs. after omT as 98]. This case report for the first time, showed the efficacy of changing microechology of otic microechology by omT. This treatment modality would be developed by the resarcher group herein dedicated.