Taurine Reduced Reproductive Performance and DNA Damage Induced by Lead in Drosophila melanogasterAbstract views: 75 / PDF downloads: 65
Keywords:Model organism, lead, taurine, pupae and reproductive development, comet assay
Lead is one of the most important pollutants in the environment and food chain. This heavy metal causes serious health risks, especially cancer, in humans and animals. Taurine is an amino acid that can be synthesized mainly from methionine and cysteine and is used especially in the food industry. This study investigated the possible protective role of taurine on reproductive performance and DNA damage in Drosophila melanogaster exposed to lead. Lead and taurine were added to the broth of D. melanogaster at 100 µM and 3 mM, respectively, for 15 days. The Comet method was used for the determination of DNA damage. It was found that there was raise in DNA damage in the lead-administered groups, whereas taurine reduced the DNA damage induced by lead. In addition, it was determined that lead caused a decrease, whereas taurine had a positive effect on reproductive performance. As a result, it was determined that taurine prevented the negative effects of lead on D. melanogaster and showed protective properties.
Acaroz DA, Ince S, Zemheri-Navruz F, Baysu-Sozbilir N. 2020. Protective effects of taurine on imidacloprid-induced DNA damage and reproductive performance in the Drosophila melanogaster model. Kocatepe Veterinary Journal, 13(2): 214-218.
Bahadorani S, Bahadorani P, Phillips JP, Hilliker AJ. 2008. The effects of vitamin supplementation on Drosophila life span under normoxia and under oxidative stress. The Journals of Gerontology Series A: Biological Sciences and Medical Sciences, 63(1): 35-42.
Bai J, Yao X, Jiang L, Zhang Q, Guan H, Liu S, Wu W, Qiu T, Gao N, Yang L, Yang G and Sun X. 2016. Taurine protects against As2O3-induced autophagy in livers of rat offsprings through PPARΥ pathway. Sci Rep, 6: 27733.
Besson MT, Ré DB, Moulin M, Birman S. 2005. High affinity transport of taurine by the Drosophila aspartate transporter dEAAT2. Journal of Biological Chemistry, 280(8): 6621-6626.
Bonacker D, Stoiber T, Böhm KJ, Prots I, Wang M, Unger E, Degen G.H. 2005. Genotoxicity of inorganic lead salts and disturbance of microtubule function. Environmental and Molecular Mutagenesis, 45(4): 346-353.
Brenneisen P, Steinbrenner H. Sies H. 2005. Selenium, oxidative stress, and health aspects. Molecular Aspects of Medicine, 26(4-5): 256-267.
Chan CY, Sun HS, Shah SM, Agovic MS, Ho I, Friedman E. Banerjee SP. 2013. Direct interaction of taurine with the NMDA glutamate receptor subtype via multiple mechanisms. Advances in Experimental Medicine and Biology, 775: 45-52.
Cohn J, Widzowski DV, Cory-Slechta DA. 1992. Lead retards development of Drosophila melanogaster. Comparative Biochemistry and Physiology Part C: Comparative Pharmacology, 102(1): 45-49.
Dhawan A, Bajpayee MM, Pandey AK, & Parmar D. (2003). Protocol for the single cell gel electrophoresis/comet assay for rapid genotoxicity assessment. Sigma, 1077(1): 1-10.
Haq R, Khan F, Haq E. (2011). Adverse effect of lead acetate on Drosophila melanogaster. Journal of Basic and Applied Sciences, 7(2): 157-163.
Hirsch HV, Mercer J, Sambaziotis H, Huber M, Stark DT, Torno-Morley T, Ruden DM. 2003. Behavioral effects of chronic exposure to low levels of lead in Drosophila melanogaster. Neurotoxicology, 24(3): 435-442.
Jimenez-Del-Rio M, Guzman-Martinez C, Velez-Pardo C. 2010. The effects of polyphenols on survival and locomotor activity in Drosophila melanogaster exposed to iron and paraquat. Neurochemical Research, 35(2): 227-238.
Jong CJ, Azuma J, Schaffer S. 2012. Mechanism underlying the antioxidant activity of taurine: prevention of mitochondrial oxidant production. Amino Acids, 42(6): 2223-2232.
Kim HM, Do CH, Lee DH. 2010. Taurine reduces ER stress in C. elegans. Journal of Biomedical Science, 17(Suppl 1): 1-6.
Kreipke RE, Kwon YV, Shcherbata HR, Ruohola-Baker H. 2017. Drosophila melanogaster as a model of muscle degeneration disorders. In: Current topics in developmental biology. Vol. 121. Academic Press. pp. 83-109.
Kumar A, Kumar A, MMS CP, Chaturvedi AK, Shabnam AA, Subrahmanyam G, Yadav KK. 2020. Lead toxicity: health hazards, influence on food chain, and sustainable remediation approaches. International journal of environmental research and public health, 17(7): 2179.
Leite GO, Ecker A, Seeger RL, Krum BN, Lugokenski TH, Fachinetto R, Sudati JH, Barbosa NV, Wagner C. 2018. Protective effect of (-)-α-bisabolol on rotenone-induced toxicity in Drosophila melanogaster. Canadian Journal of Physiology and Pharmacology. 96(4): 359-365.
Lin FJ, Pierce MM, Sehgal A, Wu T, Skipper DC, Chabba R. 2010. Effect of taurine and caffeine on sleep–wake activity in Drosophila melanogaster. Nature and science of sleep, 2: 221.
Liu ZH, Shang J, Yan L, Wei T, Xiang L, Wang HL, Xiao G. 2020. Oxidative stress caused by lead (Pb) induces iron deficiency in Drosophila melanogaster. Chemosphere, 243: 125428.
Mani MS, Kabekkodu SP, Joshi MB, Dsouza HS. 2019. Ecogenetics of lead toxicity and its influence on risk assessment. Human & Experimental Toxicology, 38(9): 1031-1059.
Morley EJ, Hirsch HV, Hollocher K, Lnenicka GA. 2003. Effects of chronic lead exposure on the neuromuscular junction in Drosophila larvae. Neurotoxicology, 24(1): 35-41.
Nagpal I, Abraham SK. 2017. Protective effects of tea polyphenols and β-carotene against γ-radiation induced mutation and oxidative stress in Drosophila melanogaster. Genes and Environment, 39: 24.
Olakkaran S, Antony A, Purayil AK, Kumbar ST., & Puttaswamygowda, G. H. (2018). Lead modulated Heme synthesis inducing oxidative stress mediated Genotoxicity in Drosophila melanogaster. Science of the Total Environment, 634:628-639.
Olive PL, Banáth JP. 2006. The comet assay: a method to measure DNA damage in individual cells. Nature Protocols, 1(1): 23-29.
Pasha Shaik A, Sankar S, Reddy SC, Das PG, Jamil K. 2006. Lead-induced genotoxicity in lymphocytes from peripheral blood samples of humans: in vitro studies. Drug and Chemical Toxicology, 29(1): 111-124.
Shilpa O, Anupama KP, Antony A, Gurushankara HP. 2021. Lead (Pb) induced oxidative stress as a mechanism to cause neurotoxicity in Drosophila melanogaster. Toxicology, 462: 152959.
Singh N, Kumar A, Gupta VK, Sharma B. 2018. Biochemical and molecular bases of lead-induced toxicity in mammalian systems and possible mitigations. Chemical Research in Toxicology, 31(10): 1009-1021.
Suh HJ, Shin B, Han SH, Woo, MJ, Hong KB. 2017. Behavioral changes and survival in melanogaster: Effects of Ascorbic acid, taurine, and caffeine. Biological and Pharmaceutical Bulletin, 40: 1873–1882.
Thier R, Bonacker D, Stoiber T, Böhm KJ, Wang M, Unger E, Degen G. 2003. Interaction of metal salts with cytoskeletal motor protein systems. Toxicology Letters, 140: 75-81.
Yang X, Zhang Z, Feng Y, Ren H, Liu F, Zu T. 2012. Effect of taurine on lifespan and antioxidant in Drosophila. In 2012 International Conference on Biomedical Engineering and Biotechnology (pp. 206-209). https://doi.org/10.1109/iCBEB.2012.170.
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