Investigation of Lipid Mobilization and Oxidative Stress Parameters in The Serum Before and After Surgery of Cows with Left Displacement Abomasum

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  • Nurcanan Aslan
  • Kürşat Yiğitarslan
  • Tülay Büyükoğlu


Dairy cow, displacement abomasum, lipid mobilization, oxidative stress


Left Abomasum Displacement (LDA) is one of the most important metabolic diseases caused by negative energy balance during the early lactation period for high milk efficient cows. This study aimed to investigate the lipid mobilization and oxidative stress parameters in cows with LDA before and after the operation. In this research, cows with LDA (n=16) were divided into three groups that are before operation (pre-op LDA), immediately after the operation (post-op LDA), and on the 10th day after the operation (post-op 10 LDA). Control groups were formed from early lactation cows (n=8) and dry period (n=8). In serum samples collected from the study groups, total cholesterol, HDL and LDL cholesterol, triacylglycerol, free fatty acid (FFA), β-hydroxybutyric acid (BHBA), malondialdehyde (MDA) levels, and AST, GGT activities were determined spectrophotometrically, total antioxidant level (TAS), total oxidant level (TOS) and paraoxonase 1/arylesterase (PON1/ARES)] enzyme activity was measured according to the procedure of the colorimetric kit. Serum TOS and MDA levels increased in cattle with pre-op LDA compared to control groups, and MDA levels decreased to normal levels in both groups after the operation. TAS levels and PON1/ARES activities decreased in cattle with pre-op LDA compared to control groups and increased gradually in post-op groups. Serum total cholesterol, HDL, and LDL cholesterol levels decreased in cattle with pre-op LDA compared to the control groups and did not return to normal levels in the groups with post-op LDA. While BHBA levels and AST activities increased in cattle with pre-op LDA compared to control groups, they reached normal values in cattle with LDA on the post-op 10th day. It has been concluded that in evaluating the diagnosis, treatment, and prognosis of the disease in dairy cows with LDA, oxidative stress parameters such as TAS, TOS, and PON1/ARES may be used together with lipid parameters.


Ahmadi M, Safi S, Mortazavi P, Rokni N. 2016. Evaluation of the enzyme changes in different grades of fatty liver syndrome in dairy cows. International Journal of Pharmaceutical Research & Allied Sciences, 5(3): 476–488.

Aly MA., Saleh NS, Allam TS, Keshta HG. 2016. Evaluation of clinical, serum biochemical and oxidant-antioxidant profiles in dairy cows with left abomasal displacement. Asian Journal of Animal and Veterinary Advances, 11(4): 242–247.

Antanaitis R, Stoškus R, Televičius M. 2015. Change of biochemical parameters in cows with abomasal displacement after omentopexy. Žemės Ūkio Mokslai, 21(4): 237–241.

Antončić-Svetina M, Turk R, Svetina A, Gereš D, Rekić B, Juretić D. 2011. Lipid status, paraoxonase-1 activity and metabolic parameters in serum of heifers and lactating cows related to oxidative stress. Research in Veterinary Science, 90(2): 298–300.

Ayar G, Atmaca YM, Alışık M, Erel Ö. 2017. Effects of paraoxonase, arylesterase, ceruloplasmin, catalase, and myeloperoxidase activities on prognosis in pediatric patients with sepsis. Clinical Biochemistry, 50(7–8): 414–417.

Celi P. 2011. Studies on Veterinary Medicine. Oxidative Stress in Applied Basic Research and Clinical Practice, in: Mandelker L, Vajdovich P. (Eds), Studies on Veterinary Medicine. Oxidative Stress in Applied Basic Research and Clinical Practice. Humana Press, Totowa, NJ, pp. 191–223.

De Koster J, Strieder-Barboza C, de Souza J, Lock AL, Contreras GA. 2018. Short communication: Effects of body fat mobilization on macrophage infiltration in adipose tissue of early lactation dairy cows. Journal of Dairy Science, 101(8): 7608–7613.

Durgut R., Sagkan Ozturk A, Ozturk OH, Guzel M. 2016. Evaluation of oxidative stress, antioxidant status and lipid profile in cattle with displacement of the abomasums. Ankara Universitesi Veteriner Fakultesi Dergisi, 63(2): 137–141.

Erel O. 2004. A novel automated method to measure total antioxidant response against potent free radical reactions. Clinical Biochemistry, 37(2): 112–119.

Erel O. 2005. A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry, 38(12): 1103–1111.

Farid AS, Honkawa K, Fath EM, Nonaka N., & Horii, Y. (2013). Serum paraoxonase-1 as biomarker for improved diagnosis of fatty liver in dairy cows. BMC Veterinary Research, 9: 73–84.

Fiore F, Spissu N, Sechi S, Cocco R. 2019. Evaluation of oxidative stress in dairy cows with left displacement of abomasum. Animals, 9(11): 1–9.

Folnožić I, Turk R, Duričić D, Vince S, Pleadin J, Flegar-Meštrić Z, Valpotić H, Dobranić T, Gračner D, Samardžija M. 2015. Influence of body condition on serum metabolic indicators of lipid mobilization and oxidative stress in dairy cows during the transition period. Reproduction in Domestic Animals, 50(6): 910–917.

Friedewald WT, Levy RI, Fredrickson DS. 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinical Chemistry, 18(6), 499–502.

Fürll M, Dabbagh M, Kirbach H, Nauruschat C, Sattler T, Wilken H. 2003. The anti-oxidative state before and after a diagnosed abomasal displacement in cows. Acta Veterinaria Scandinavica, 44(Suppl 1), P37.

Ghazy A, Gomaa N, Nasr N. 2016. Hematological and biochemical evaluation in Holstein-Friesian cows before and after surgical correction of left abomasal displacement on-filed condition. Alexandria Journal of Veterinary Sciences, 49(1): 138.

Guzelbektes H, Sen I, Ok M, Constable PD, Boydak M, Coskun A. 2010. Serum amyloid a and haptoglobin concentrations and liver fat percentage in lactating dairy cows with abomasal displacement. Journal of Veterinary Internal Medicine, 24(1): 213–219.

Haagen L, Brock A. 1992. A new automated method for phenotyping arylesterase (ec based upon ınhibition of enzymatic hydrolysis of 4-nitrophenyl acetate by phenylacetate. Clinical Chemistry and Laboratory Medicine, 30(7): 391–396.

Hasanpour A, Saranjam N, Tabrizi BA. 2011. Antioxidant concentration status in the serum of cows with left displacement abomasum. Global Veterinaria, 7(5): 478–481.

Imhasly S, Naegeli H, Baumann S, von Bergen M, Luch A, Jungnickel H., Potratz S, Gerspach C. 2014. Metabolomic biomarkers correlating with hepatic lipidosis in dairy cows. BMC Veterinary Research, 10(1): 122–130.

Katoh N. 2002. Relevance of apolipoproteins in the development of fatty liver and fatty liver-related peripartum diseases in dairy cows. Journal of Veterinary Medical Science, 64(4): 293–307.

Khalphallah A, Aamer A, Oikawa S, Nakada K, AbdelAll T, Katoh H, Elmeligy E. 2016. Diagnostic and prognostic significance of lipid profiles in Holstein dairy cattle with displaced abomasum : before and after surgical operation. Journal of Advanced Veterinary Research, 6(1): 7–17.

Klevenhusen F, Humer E, Metzler-Zebeli B, Podstatzky-Lichtenstein L, Wittek T, Zebeli Q. 2015. Metabolic profile and inflammatory responses in dairy cows with left displaced abomasum kept under small-scaled farm conditions. Animals, 5(4): 1021–1033.

Kulka M, Kołodziejska-Lesisz J, Kluciński W. 2016. Serum paraoxonase 1 (PON1) activity and lipid metabolism parameters changes in different production cycle periods of Holstein-Friesian, Polish Red and Norwegian breeds. Polish Journal of Veterinary Sciences, 19(1): 165–173.

LeBlanc S. 2010. Monitoring metabolic health of dairy cattle in the transition period. Journal of Reproduction and Development, 56(SUPPL.), 29–35.

LeBlanc SJ, Leslie KE, Duffield TF. 2005. Metabolic predictors of displaced abomasum in dairy cattle. Journal of Dairy Science, 88(1), 159–170.

Locher L, Sattler T, Wittek T. 2011. Die bedeutung, erhebung und bewertung des antioxidativen status bei landwirtschaftlichen nutztieren. Berliner Und Munchener Tierarztliche Wochenschrift, 124(9–10): 419–431.

Maden M, Ozturk AS, Bulbul A, Avci GE, Yazar E. 2012. Acute-phase proteins, oxidative stress and enzyme activities of blood serum and peritoneal fluid in cattle with abomasal displacement. Journal of Veterinary Internal Medicine, 26(6): 1470–1475.

Mamak N, Devrim AK, Aksit H, Aytekin I, Yildiz R. 2013. Levels of antioxidant substances, acute phase response and lipid peroxidation in the left and right abomasum displacement in cows. Polish Journal of Veterinary Sciences, 16(4): 731–733.

Markiewicz H, Gehrke M, Malinowski E. 2009. Selected biochemical blood compounds in cows with abomasum displacement. Polish Journal of Veterinary Sciences, 12(4): 515–518.

McArt JAA, Nydam DV, Oetzel GR, Overton TR, Ospina PA. 2013. Elevated non-esterified fatty acids and β-hydroxybutyrate and their association with transition dairy cow performance. Veterinary Journal, 198(3), 560–570.

Mokhber Dezfouli M., Eftekhari Z, Sadeghian S, Bahounar A, Jeloudari M. 2013. Evaluation of hematological and biochemical profiles in dairy cows with left displacement of the abomasum. Comparative Clinical Pathology, 22(2): 175–179.

Mudron P, Rehage J, Sallmann HP, Mertens M, Scholz H, Kovac G. 1997. Plasma and Liver α-tocopherol in Dairy Cows with Left Abomasal Displacement and Fatty Liver. Journal of Veterinary Medicine Series A: Physiology Pathology Clinical Medicine, 44(2): 91–97.

Puppel K, Kuczyńska B. 2016. Metabolic profiles of cow’s blood; a review. Journal of the Science of Food and Agriculture, 96(13): 4321–4328.

Qu Y, Lytle K, Traber MG, Bobe G. 2013. Depleted serum vitamin E concentrations precede left displaced abomasum in early-lactation dairy cows. Journal of Dairy Science, 96(5): 3012–3022.

Reitman S, Frankel S. 1957. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. American Journal of Clinical Pathology, 28(1): 56–63.

Satoh K. 1978. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clinica Chimica Acta, 90(1): 37–43.

Sevinc M, Ok M, Basoglu A. 2002. Liver function in dairy cows with abomasal displacement. Revue de Medecine Veterinaire, 153(7): 477–480.

Sezer K, Kabu M, Yiğitarslan K, Karakurum MÇ. 2012. Comparison of some biochemical parameters in dairy cows with abomasal displacement at the pre-and post-operative stages. Fırat University Veterinary Journal of Health Sciences, 26(3): 175–181.

Tian W, Wei ., Li B, Wang Z, Zhang N, Xie G. 2014. Pathway of programmed cell death and oxidative stress induced by β-hydroxybutyrate in dairy cow abomasum smooth muscle cells and in mouse gastric smooth muscle. PLoS ONE, 9(5): 1–12.

Turk R, Juretić D, Gereš D, Svetina A, Turk N, Flegar-Meštrić Z. 2008. Influence of oxidative stress and metabolic adaptation on PON1 activity and MDA level in transition dairy cows. Animal Reproduction Science, 108(1–2): 98–106.

Turk R, Juretic D, Geres D, Turk N, Rekic B, Simeon-Rudolf V, Svetina A. 2004. Serum paraoxonase activity and lipid parameters in the early postpartum period of dairy cows. Research in Veterinary Science, 76(1): 57–61.

Turk R, Podpečan O, Mrkun J, Kosec M, Flegar-Meštrić Z, Perkov S., Starič J, Robić M, Belić M, Zrimšek, P. 2013. Lipid mobilization and oxidative stress as metabolic adaptation processes in dairy heifers during transition period. Animal Reproduction Science, 141(3–4): 109–115.

Van Winden SCL, Jorritsma R, Müller KE, Noordhuizen JPTM. 2003. Feed intake, milk yield, and metabolic parameters prior to left displaced abomasum in dairy cows. Journal of Dairy Science, 86(4): 1465–1471.

Warnick GR, Nguyen T, Albers AA. 1985. Comparison of improved precipitation methods for quantification of high-density lipoprotein cholesterol. Clinical Chemistry, 31(2): 217–222.

Wilson DG. 2008. Management of abomasal displacement. Large Animal Veterinary Rounds, 8(8): 1–6.

Yagi K. 1984. Assay for Blood Plasma or Serum. Methods in Enzymology, 105(C): 328–331.

Yildiz R, Ider M, Ok M. 2019. Beta hidroksi bütirik asit düzeyinin diğer metabolik test parametreleri üzerine etkisi. Veteriner Hekimler Derneği Dergisi, 90(1), 15–21.

Zak B, Luz DA, Fisher M. 1957. Determination of serum cholesterol. The American Journal of Medical Technology, 23(5): 283–287.




How to Cite

Aslan, N., Yiğitarslan, K. ., & Büyükoğlu, T. (2022). Investigation of Lipid Mobilization and Oxidative Stress Parameters in The Serum Before and After Surgery of Cows with Left Displacement Abomasum. International Journal of Veterinary and Animal Research (IJVAR), 5(2), 80–88. Retrieved from



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