Investigation of Vancomycin Resistance and Some Virulence Factors in Enterococci Strains Isolated from Dogs

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Authors

  • Özgül Gülaydın
  • İsmail Hakkı Ekin
  • Ziya Ilhan
  • Muazzez Yesilyurt

Keywords:

Antimicrobial susceptibility, dog, E. faecalis, E. faecium, vancomycin, virulence gene

Abstract

The aim of this study was to evaluate in vitro antimicrobial susceptibility of Enteroccoccus faecalis and Enterococcus faecium strains isolated from dog, as well as the phenotypic and genotypic characterization of vancomycin resistance and some virulence genes of the isolates. A total of 197 samples were analyzed, including 114 urine, 63 rectal swab and 20 vaginal swab samples. Enteroccocus spp. were isolated from 83 (42.1%) of the samples. By PCR test with species-specific primers, 42 (50.6%) isolates were identified as E. faecalis (95.2%) and E. faecium (4.8%). The highest resistance was found to erythromycin (16.66%) and tetracycline (11.9%) whereas vancomycin resistance was low (4.76%) by disc diffusion method. GelE (47.6%) and asa1 (38.1%) genes associated with the virulence were detected in the isolates, while all isolates were negative for cylA gene. As a result, E. faecalis from canine samples was isolated at higher rate than that of E. faecium, and the resistance of the isolates to β-lactam antibiotics (ampicillin, penicillin, imipenem, and vancomycin) as well as multiple antibiotic resistance was low. The gelE gene was detected in more isolates than the asa1 gene, and all isolates were negative for cylA gene.

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Published

2022-08-27

How to Cite

Gülaydın, Özgül, Ekin, İsmail H., Ilhan, Z., & Yesilyurt, M. (2022). Investigation of Vancomycin Resistance and Some Virulence Factors in Enterococci Strains Isolated from Dogs. International Journal of Veterinary and Animal Research (IJVAR), 5(2), 54–59. Retrieved from https://ijvar.org/index.php/ijvar/article/view/537

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Research Articles